Journal of Food and Nutrition Sciences - No.3, December, Year 2003
Author: Le Hong Dung
English summary:
The aim of this research is to study the antimutagenic activity of sticky rice cooked with various fruits vegetables and legumes. Fruits and vegetables rich in carotenoids including ivy gourd leaves, the fruit from Momordica cochinchinensis and pumpkin, two kind of legumes – mungbean and black bean – and sticky rice colored by being cooked with these fruits, vegetables and legumes were sequentially extracted with n-hexane and 80% ethanol. The extracted materials were tested for inhibition of mutagenicity induced by direct –acting mutagen generated from nitrite – treated 1 – aminopyrene in Salmonella typhimurium TA98 and TA100. Antimutagenic activities were found in all extracts on both strains TA98 and TA100. The highest activities were found in extracts from ivy gourd leaves and sticky rice cooked with these leaves, with maximal percentage inhibition of 84.7 – 96.6% in TA98 and 76.0 -91.5% in TA100. The hexane fraction expressed higher activity than the ethanol fraction among extracts from ivy gourd leaves,momordica cochinchinensis fruit and pumpkin, and active compounds were suggested for carotenoids and cholrophyll. Whilst among ethanol extracts, those from black bean and mungbean showed the highest activities,and active compounds were suggested for phenolic compounds. With respect to cooking effect on Antimutagenic, an increase in activity after cooking was found in the case of ivy gourd leaves, Momordica cochinchinensis fruit and pumpkin. In contrast, a decrease in antimutagenic was found in the case of mungbean and black bean.
Keyword: Genetic mutations, Salmonella Typhimurium TA98 and TA100.